Brown adipose tissue (BAT) activity is influenced by the outdoor temperature variation. However, people spend most of their time indoors, especially in colder regions and during cold seasons. Therefore, outdoor temperature is probably not an accurate tool to quantify the exposure of the participants before BAT quantification. We studied the association of wrist and personal environmental temperatures with cold-induced BAT and skeletal muscle [F-18]fluorodeoxyglucose ([F-18]FDG) uptake in adults. A total of 74 participants wore two iButtons during 7 days to measure wrist temperature (WT) and personal level of environmental temperature (Personal-ET). Thereafter, we performed a 2-h personalized cooling protocol before performing an [F-18]FDG-PET/CT scan. WT was negatively associated with BAT volume (R-2 = 0.122; P = 0.002) and BAT activity [standardized uptake value (SUV)(peak,) R-2 = 0.083; P = 0.012]. Moreover, Personal-ET was negatively associated with BAT volume (R-2 = 0.164; P 0.001), BAT activity (SUVmean and SUVpeak, all R-2 >= 0.167; P < 0.001). and skeletal muscle activity (SUVpeak, R-2 = 0.122; P = 0.002). Interestingly, the time exposed to a certain Personal-ET (16-20 degrees C) positively corre-lated only with [F-18]FDG uptake by BAT (volume and activity; all P <= 0.05). whereas the time exposed to 12-15 degrees C positively correlated only with measures of [ 18 F]FDG uptake by skeletal muscle activity (all P <= 0.05). This study shows that WT and Personal-ET are associated with [F-18]FDG uptake by BAT and skeletal muscle activity in adults within certain temperature thresholds. Moreover, our results suggest that [F-18]FDG uptake by human BAT or skeletal muscle can be activated or inhibited in different ranges of ambient temperatures exposures. Results should be taken with caution because the observed associations were relatively weak.