Publicación

MICROWAVE-OVEN DIGESTION PROCEDURE FOR ATOMIC-ABSORPTION SPECTROMETRY ANALYSIS OF BONE AND TEETH

  • CLINICA CHIMICA ACTA
  • Autores
    GIL, F; PEREZ, ML; FACIO, A; VILLANUEVA, E; TOJO, R; GIL, A
  • Año Publicación
    1993
  • Volumen
    221
  • Número
    1-Feb
  • Pág. Inicio
    23
  • Pág. Fin
    31
  • Pág. Fin
    23
Referencia Citadas
20
Citas Web of Science
18
Total de veces citado (Z9)
18
Recuento Uso 180 días
2
Recuento Uso 5 años
8

The purpose of this study was to develop a new method for determination of the mineral element in teeth and bone using a microwave digestion procedure coupled to atomic absorption spectrometry. Samples were ashed at 450 degrees C for 4.5 h and subsequently digested in a microwave oven at maximum power (600 W) for 10 s using a Teflon closed Parr bomb. This method permits the rapid dissolution of the biological samples, avoiding operations such as slicing and cutting which often introduce alterations in sample homogeneity and may contribute to their contamination. The analysis of Na, K, Ca, Mg, Fe, Zn, Ba, Cd and Pb showed good reproducibility, precision and accuracy as compared with the certified values fdr the IAEA H-5 animal bone. Average concentration for those elements in permanent teeth obtained from children aged 11-18 years by orthodontics are given.


Web financiada por la Junta de Andalucía, Consejería de Conocimiento, Investigación y Universidades, Fondo Europeo de Desarrollo Regional (FEDER), proyecto SOMM17/6107/UG

Web financiada por la Junta de Andalucía, Consejería de Conocimiento, Investigación y Universidades, Fondo Europeo de Desarrollo Regional (FEDER), proyecto SOMM17/6107/UGR