This study aimed to investigate the effects of different training modalities on Klotho protein plasma levels (S-Klotho) in sedentary middle-aged adults. 

Seventy-four individuals were randomly assigned to 4 different groups: (i) control (no exercise), (ii) physical activity recommendation from the World Health Organization group, (iii) high intensity interval training (HIIT), and (iv) a HIIT + whole-body electromyostimulation.

All exercise training modalities induced an increase in S-Klotho. Moreover, a positive association was observed between changes in lean mass and changes in S-Klotho plasma levels, whereas a negative association was reported between changes in fat mass outcomes and changes in S-Klotho.